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Cell Insert

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Membrane:
  
PC Membrane Cell Insert, Tissue Culture Treated
Cat.No. Type
(Well)
Pore Size
(um)
Membrane Density Insert Membrane Growth Area(cm2) Insert Diameter
(mm)
Well Volume
(ml)
Insert Volume
(ml)
packing specification
723101 6 0.4 1 x 108 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724101 12 0.4 1 x 108 well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725101 24 0.4 1 x 108well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
723001 6 3 2 x 106 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724001 12 3 2 x  106well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725001 24 3 2 x  106well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
724201 12 5 4 x  105well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725201 24 5 4 x  105well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
723301 6 8 1 x 105 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724301 12 8 1 x105well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725301 24 8 1 x105well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
PC Membrane Cell Insert, Non-treated
Cat.No. Type
(Well)
Pore Size
(um)
Membrane Density Insert Membrane Growth Area(cm2) Insert Diameter
(mm)
Well Volume
(ml)
Insert Volume
(ml)
packing specification
723111 6 0.4um 1 x 108 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724111 12 0.4um 1 x108 well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725111 24 0.4um 1 x 108 well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
723011 6 3um 2 x106 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724011 12 3um 2 x 106 well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725011 24 3um 2 x 106well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
724211 12 5um 4 x  105well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725211 24 5um 4 x  105well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
723311 6 8um 1 x 105 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724311 12 8um 1 x 105 well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725311 24 8um 1 x 105 well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
50% Transparent PC Membrane Cell Insert, Tissue Culture Treated
Cat.No. Name Pore Size
(um)
Membrane Density Insert Membrane Growth Area(cm2) Insert Diameter
(mm)
Well Volume
(ml)
Insert Volume
(ml)
packing specification
726001 100mm Dish 3um 2 x 106 well/cm2 44 75mm 13ml 9ml 10 inserts/case

Transparent PET Membrane Cell Insert, Tissue Culture Treated
Cat.No. Type
(Well)
Pore Size
(um)
Membrane Density Insert Membrane Growth Area(cm2) Insert Diameter
(mm)
Well Volume
(ml)
Insert Volume
(ml)
packing specification
723121 6 0.4 1 x 108 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724121 12 0.4 1 x 108 well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725121 24 0.4 1 x 108 well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
723021 6 3 2 x 106 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724021 12 3 2 x  106well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725021 24 3 2 x  106 well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case

Transparent PET Membrane Cell Insert, Non-treated
Cat.No. Type
(Well)
Pore Size
(um)
Membrane Density Insert Membrane Growth Area(cm2) Insert Diameter
(mm)
Well Volume
(ml)
Insert Volume
(ml)
packing specification
723131 6 0.4 1 x 108 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724131 12 0.4 1 x 108 well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725131 24 0.4 1 x 108 well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case
723031 6 3 2 x 106 well/cm2 4.67 24 2.6 1.5 6 inserts/6well plate 60 inserts/case
724031 12 3 2 x 106 well/cm2 1.12 12 1.5 0.5 12 inserts/12well plate 120 inserts/case
725031 24 3 2 x 106 well/cm2 0.33 6.5 0.6 0.1 12 inserts/24well plate 120 inserts/case



Multiple well plate  or dish is made from PS


Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines. 
This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.
Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.



·Innovative hanging design facilitates pipetting.
·Supplied in multiple well plates: 6-well, 12-well, 24well.
·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
·Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy. 
· Transparent PET membrane is easy to observe.
·The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
·Compatible with most solvents used for fixing and dyeing.

Attention:
? Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
?During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
?Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines for us:
? Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. ? Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
? Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
? Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
? Check the volume of the culture medium regularly and add fresh medium as needed.
? The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
? Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.



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